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Methodologic and biological variability of quantitative real-time polymerase chain reaction analysis of Bcr-Abl expression in Philadelphia chromosome-positive acute lymphoblastic leukemia

Scheuring, U.J.; Pfeifer, H.; Wassmann, B.; Brück, P.; Hoelzer, D.; Ottmann, O.G.

Haematologica 88(9): 1074-1076

2003

ISSN/ISBN: 0390-6078
PMID: 12969819
Document Number: 552839
We examined to what extent technical and biological factors may affect the validity of Bcr-AbI polymerase chain reaction quantification in Philadelphia chromosome-positive acute lymphocytic leukemia and found that technical variance is the predominant limitation of the method and was not exceeded by biological variance. Interestingly, the number of p210Bcr-AbI (b2a2 or b3a2) transcripts per blast was more then 10-fold higher than the number of p190Bcr-AbI copies per blast.

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