Top Abstract Purchase Related
Home
>
Node
>
Subnode

Overexpression of Escherichia coli single stranded DNA binding protein under bacteriophage T 7 promoter

Kishore, U.; Chauhan, M.; Choudhury, N.R.; Khatri, G.S.

Indian Journal of Biochemistry and Biophysics 31(4): 249-253

1994

ISSN/ISBN: 0301-1208
PMID: 8002005
Document Number: 429681
A recombinant vector for overproduction of the E. coli single stranded DNA binding protein (E. coli SSBP) has been constructed. An E. coli strain carrying this plasmid produces up to 150 mg pure SSBP per litre of bacterial culture in a laboratory shake flask. Electron microscopy of the single stranded DNA complexed with SSBP shows characteristic "beaded string"-like appearance. Strong clustering of protein molecules on ssDNA is indicative of a highly cooperative binding.

Document emailed within 1 workday
Secure & encrypted payments

Stepanenko, O.'g.V.; Povarova, O.I.; Fonin, A.V.; Stepanenko, O.V. 2010: Stability of sugar-binding proteins: D-galactose/D-glucose-binding protein from Escherichia coli and trehalose/maltose-binding protein from Thermococcus litoralis Tsitologiia 52(11): 950-954
Ishchenko, A.A.; Bulychev, N.V.; Maksakova, G.A.; Johnson, F.; Nevinskiĭ, G.A. 1998: Interaction of Escherichia coli 8-oxoguanine DNA glycosylase with single-stranded oligodeoxyribonucleotides and their complexes Molekuliarnaia Biologiia 32(3): 549-558
Zlochevskiĭ, M.L.; Kozlov, I.I.; Debabov, V.G. 1978: Complex of single-stranded phage DNA and protein--a product of bacteriophage fl gene 3. Distribution of gene 3 protein in the DNA molecule Molekuliarnaia Biologiia 12(3): 505-514
Dzhataev, S.A.; Tanirbergenov, T.B.; Tarasov, V.A. 1980: Formation of single-stranded breaks in newly-synthesized Escherichia coli DNA following treatment with bleomycin Genetika 16(4): 733-735
Gabig, M.; Obuchowski, M.; Ciesielska, A.; Latała, B.; Wegrzyn, A.; Thomas, M.S.; Wegrzyn, G. 1998: The Escherichia coli RNA polymerase alpha subunit and transcriptional activation by bacteriophage lambda CIi protein Acta Biochimica Polonica 45(1): 271-280
Mensa-Wilmot, K.; Carroll, K.; McMacken, R. 1989: Transcriptional activation of bacteriophage lambda DNA replication in vitro: regulatory role of histone-like protein HU of Escherichia coli EMBO Journal 8(8): 2393-2402
Koterov, A.N. 1997: Effect of zinc-metallothionein on DNA replicative synthesis and single-stranded DNA-binding protein (SSB-protein) content in chromatin of mice bone marrow cells Ukrainskii Biokhimicheskii Zhurnal 69(1): 84-87
Blok, L.N.; Rozenberg, V.D.; Fel'dman, E.E.; Anokhina, G.A. 1973: RNA and protein synthesis in the presence of DNA and DNP of different origin in a DNA-directed cell-free system from Escherichia coli B extracts following bacteriophage T2 infection Ukrains'kyi Biokhimichnyi Zhurnal 45(2): 136-141
Spassky, A.; Busby, S.; Buc, H. 1984: On the action of the cyclic AMP-cyclic AMP receptor protein complex at the Escherichia coli lactose and galactose promoter regions EMBO Journal 3(1): 43-50
Yazawa, M.; Yagi, K. 1989: Enzyme activation by yeast calmodulin and a calcium-binding protein of Escherichia coli Progress in Clinical and Biological Research 315: 458-459
Puspurs, A.H.; Trun, N.J.; Reeve, J.N. 1983: Bacteriophage Mu DNA circularizes following infection of Escherichia coli EMBO Journal 2(3): 345-352
De Lafonteyne, J. 1971: Breakdown of bacteriophage lambda DNA in "Escherichia coli" K12 "rec." bacteria Archives Internationales de Physiologie et de Biochimie 79(3): 629-630
Van de Voorde, A.; Fiers, W. 1971: Virus-specific RNA synthesis in bacteriophage MS2-infected "Escherichia coli" Archives Internationales de Physiologie et de Biochimie 79(3): 638-639
Berzin', V.M.; Gren, E.I. 1972: Synthesis of stable RNAs in Escherichia coli cells infected with MS2 bacteriophage Biokhimiia 37(4): 874-876
Ozolin', O.N.; Deev, A.A.; Masulis, I.S.; Chasov, V.V.; Kostianitsyna, E.G.; Purtov, I.A.; Arkhipov, I.V.; Brok-Volchanskiĭ, A.S. 2002: Levels in the structural organization of Escherichia coli promoter DNA Biofizika 47(5): 809-819
De Mey, M.; Van Nieuland, K.; Vandamme, E.J. 2004: Development of an artificial promoter library for Escherichia coli Communications in Agricultural and Applied Biological Sciences 69(2): 93-96
Upmal, M.R.; Kobets, N.S.; Il'ina, T.S.; Piruzian, E.S. 1984: Genetic analysis of the Mud mutants of Escherichia coli K-12 with an impaired ability to maintain bacteriophage Mu development Genetika 20(6): 924-932
Kanaya, S.; Kudo, Y. 1991: Specificity of promoter consensus sequences in Escherichia coli chromosome Nucleic Acids Symposium Series 1991(25): 41-42
García-Quintana, H.; Imchenetzky, M.; Wilckens, R.; Polette, M. 1980: First natural population of Escherichia coli bacteriophage isolated in Chile. V. Biochemical properties of the virus ZAR2 Revista Medica de Chile 108(10): 893-899
Oethinger, M.; Podglajen, I.; Kern, W.V.; Levy, S.B. 1998: Overexpression of the marA or soxS regulatory gene in clinical topoisomerase mutants of Escherichia coli Antimicrobial Agents and Chemotherapy 42(8): 2089-2094