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Identification of testis specific calcineurin beta subunit isoform by a monoclonal antibody and detection of a specific six amino acid sequence

Nishio, H.; Matsui, H.; Etoh, S.; Moia, L.J.; Tokuda, M.; Itano, T.; Hatase, O.

Biochemical and Biophysical Research Communications 182(1): 34-38

1992

ISSN/ISBN: 0006-291X
PMID: 1310015
Document Number: 390930
Two isoforms of calcineurin .beta. subunit (.beta.1 and .beta.2) were identified in rat testis by a monoclonal antibody Va1. Both .beta.1 and .beta.2 were recovered in calmodulin binding protein fraction and showed calcium shift on SDS-polyacrylamide gel electrophoresis which is the specific character for EF-hand calcium binding protein. .beta.2 showed same apparent molecular weight on SDS-PAGE as that of brain calcineurin .beta. and was found in wide variety of tissues. .beta.1 was shown to have six amino acid polypeptide sequence and it showed higher molecular weight that brain .beta. and was specific for testis.

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