Evaluation of total protein content in tears of dogs by polyacrylamide gel disk electrophoresis
Barrera, R.; Jiménez, A.; López, R.; Mañé, M.C.; Rodríguez, J.F.; Molleda, J.M.
American Journal of Veterinary Research 53(4): 454-456
1992
ISSN/ISBN: 0002-9645 PMID: 1586012 Document Number: 390653
Concentration of total proteins was measured and sodium dodecyl sulfate-polyacrylamide gel disk electrophoresis was performed on tear and plasma samples obtained from 26 healthy dogs, and the results were compared. Mean .+-. SEM concentrations of total proteins in tears was 0.63 .+-. 0.04 g/dl, and significant effects of age or gender were not found. The protein composition of tears in dogs was complex, and bands from light and heavy chains of immunoglobulins were identified by electrophoresis.