Cytological observation on culture of embryo in vitro of Tripterygium hypoglaucum (level.) Hutch
Guo, X.; Yang, M.
Zhongguo Zhong Yao Za Zhi 17(6): 332-336; 381-382
1992
ISSN/ISBN: 1001-5302 PMID: 1418574 Document Number: 390119
This paper for the first time reports the embryo culture in vitro of Tripterygium hypoglaucum. Callus, adventitious roots and adventitious protuberances were induced from the cultured embryos in vitro. Multi-buds at the excised embryo tip were also observed at the same time. Cytohistological examination showed (1) callus was histologically easy to be induced to form adventitious roots; (2) callus on the cotyledons was originated from the epidemical and subepidemical cells of the cotyledons, and the adventitious roots were originated from the disperse meristematic tissue or vascular tissue in the callus obtained from cultured excised embryos.