Separation of glutathione S-transferase activities with epoxides from the mouse liver h-protein, a major polycyclic hydrocarbon-binding protein
Sarrif, A.M.; McCarthy, K.L.; Nesnow, S.; Heidelberger, C.
Cancer Research 38(5): 1438-1443
1978
ISSN/ISBN: 0008-5472 PMID: 416909 Document Number: 134945
The C3H mouse liver h-protein is a cytoplasmic protein to which metabolites of carcinogenic polycyclic hydrocarbons bind covalently following i.p. injection. It has a number of physical properties similar to those of the glutathione S-transferases (EC 2.5.1.18). These properties include molecular weight (40,000), number of subunits (2), basic isoelectric point around 8.0, sedimentation coefficient (3.5S), and subcellular localization. In this communication, we have shown that glutathione S-transferase activities with 1,2-epoxy(3-p-nitrophenoxy)propane and benzanthracene conjugate or as the free form is also described.