Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Meta-Analysis
. 2014:2014:573783.
doi: 10.1155/2014/573783. Epub 2014 Jul 16.

K-ras mutational status in cytohistological tissue as a molecular marker for the diagnosis of pancreatic cancer: a systematic review and meta-analysis

Jing Yang  1 , Jingjing Li  1 , Rong Zhu  1 , Huawei Zhang  1 , Yuanyuan Zheng  1 , Weiqi Dai  1 , Fan Wang  1 , Miao Shen  1 , Kan Chen  1 , Ping Cheng  1 , Yan Zhang  1 , Chengfen Wang  1 , Junshan Wang  1 , Yujing Xia  1 , Jie Lu  1 , Yingqun Zhou  1 , Chuanyong Guo  1
Affiliations
Meta-Analysis

K-ras mutational status in cytohistological tissue as a molecular marker for the diagnosis of pancreatic cancer: a systematic review and meta-analysis

Jing Yang et al. Dis Markers. 2014.

Abstract

Background: More clinically meaningful diagnostic tests are needed in pancreatic cancer (PC). K-ras mutations are the most frequently acquired genetic alteration.

Methods: Original research articles involving the diagnostic accuracy of K-ras mutation detection in PC were selected. Data were presented as forest plots and summary receiver operating characteristic (SROC) curve analysis was used to summarize the overall test performance.

Results: We assessed 19 studies from 16 published articles. The reports were divided into three groups according to the process used to obtain the test material. The summary estimates for detecting K-ras status using an invasive method (fine needle aspiration (FNA), endoscopic retrograde cholangiopancreatography (ERCP), or surgery) were better than cytology: the pooled sensitivity was 77% (95% confidence interval (CI): 74-80%) versus 54% (95% CI: 47-61%); specificity was 88% (95% CI: 85-91%) versus 91% (95% CI: 83-96%); and diagnostic odds ratio (DOR) was 20.26 (11.40-36.03) versus 7.52 (95% CI: 2.80-20.18), respectively. When two procedures were combined, the diagnostic accuracy was markedly improved.

Conclusions: The analysis of K-ras mutations in pancreatic tissue has a promising diagnostic significance in PC. Further valuable studies are needed.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Flow diagram showing the process of article selection for the meta-analysis.
Figure 2
Figure 2
Summary of the methodological quality of included studies on the basis of review authors' judgments on the four domains of the QUADAS-2 checklist for each study.
Figure 3
Figure 3
Forest plot of the meta-analysis of each index.
Figure 4
Figure 4
Summary receiver-operating characteristic curves for detecting K-ras mutation using FNA/ERCP/surgical methods from the hierarchical summary receiver operating characteristic model.
Figure 5
Figure 5
Forest plot of the meta-analysis of seven studies which included the diagnostic value of cytology for detecting PC.
Figure 6
Figure 6
Deeks funnel plot output using the “metafunnel” command for Stata (version 12.0).
See this image and copyright information in PMC

References

    1. Warshaw AL, Fernandez-del Castillo C. Pancreatic carcinoma. New England Journal of Medicine. 1992;326(7):455–465. - PubMed
    1. Rosty C, Goggins M. Early detection of pancreatic carcinoma. Hematology/Oncology Clinics of North America. 2002;16(1):37–52. - PubMed
    1. Oto A, Eltorky MA, Dave A, et al. Mimicks of pancreatic malignancy in patients with chronic pancreatitis: correlation of computed tomography imaging features with histopathologic findings. Current Problems in Diagnostic Radiology. 2006;35(5):199–205. - PubMed
    1. Fry LC, Mönkemüller K, Malfertheiner P. Molecular markers of pancreatic cancer: development and clinical relevance. Langenbeck's Archives of Surgery. 2008;393(6):883–890. - PubMed
    1. Almoguera C, Shibata D, Forrester K, Martin J, Arnheim N, Perucho M. Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes. Cell. 1988;53(4):549–554. - PubMed

Publication types

Substances

LinkOut - more resources