To conclude, while pyrene units at the tandem junction are likely to cause destabilization of corresponding duplexes with
complementary DNA target, an attendant effect of stabilization caused by hydrophobic interactions between multiple pyrene units at the junction resulted in difference of only 2.1[degrees]C between [T.sub.m] values of duplexes (IVb+IIb) x fcDNA and (IV+II) x fcDNA.
In a statement issued after the decision was announced, the company noted that the Court "upheld its patents on
complementary DNA....
However, nobody had succeeded in cloning a full-length [alpha]-globin
complementary DNA (cDNA) copy of the mRNA.
The researchers assembled snippets of genetic code from healthy human prostate tissue into a
complementary DNA (cDNA) library.
In the present study, we included
complementary DNA nucleotide sequences for each gene.
As DNA microarrays have evolved from in-house
complementary DNA arrays to robust commercial oligonucleotide platforms, there has been a migration to higher density biochips with increasing content and better analytical methodologies.
Thus, the enzyme requires a single strand for a template and a small RNA fragment for the synthesis of a
complementary DNA strand.
As a result of morphological examination and
complementary DNA analyses, these 5 species are considered synonyms of S.
This homogeneous phase detection assay is based on the simultaneous binding of 2 aptamer-containing oligonucleotides, each linked to a
complementary DNA reporter sequence containing 2 reactive fluorophore precursor molecules.
RNA was extracted from 250 [micro]L virus-infected culture supernatant by using Trizol LS (Invitrogen, Carlsbad, CA, USA), and the
complementary DNA was synthesized with Oligo(dT)15 (Invitrogen) by using SuperScriptII reverse transcriptase (Invitrogen).
Under normal circumstances, nucleic acid sequences that have large stretches of G residues have a tendency to bind together instead of binding to their
complementary DNA strand, making those sequences extremely difficult to design assays to detect.
mRNA are converted into
complementary DNA (cDNA) and labeled red (infected) and green (noninfected).
At the end of the treatment periods, mRNA was extracted from the cells, pooled according to treaunent and cell line, and used to create labeled
complementary DNA samples.
