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Comment: Re: raw intensities in affymetrix .cel files by Aswet • 0

The passage highlights that the Statistical Algorithms used in GeneChip analysis rely on data already present in the .CEL files, which are …

Comment: Limma and Affymetrix ? by Baltex • 0

For Limma with Affymetrix data, create a design matrix for your factors, fit the model using lmFit(), and define your contrasts with makeCo…

Comment: limpa for protein quantification & differential expression analysis: Accounting by Gordon Smyth 53k

I have edited my answer above now to explain how to average technical replicates.

Answer: edgeR: Clustering for heatmap on the means of group instead of replicates by Gordon Smyth 53k

edgeR provides the function `cpmByGroup()` to compute mean expression per group, and the recommended way to get logCPM by group is to run t…

Comment: combine two objects & adding/deleting arrays in an object by Baltex • 0

To combine two objects of the same class (e.g., two marrayRaw objects), you typically merge their internal data using functions like c() or…

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Popular Question to Dario Strbenac ★ 1.6k

Teacher to Gordon Smyth 53k