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. 1988 Oct 31;177(3):245-52.
doi: 10.1016/0009-8981(88)90069-1.

Accurate and specific HPLC assay of coproporphyrinogen III oxidase activity in human peripheral leucocytes

R Guo  1 , C K Lim, T J Peters
Affiliations

Accurate and specific HPLC assay of coproporphyrinogen III oxidase activity in human peripheral leucocytes

R Guo et al. Clin Chim Acta. .

Abstract

An HPLC method is described for the assay of coproporphyrinogen III oxidase in human leucocytes. The optimal pH for the assay was 6.5-7.0 and the Km for coproporphyrinogen III was 0.12 +/- 0.021 mumol/l. The mean activity in 28 apparently health subjects was 0.249 (2 SD range 0.130-0.368) nmol/h per mg protein. In two patients with hereditary coproporphyria, the activities were 0.029 and 0.078 nmol/h per mg protein.

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