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. 2005 Jun;187(11):3855-8.
doi: 10.1128/JB.187.11.3855-3858.2005.

Structure and genome organization of AFV2, a novel archaeal lipothrixvirus with unusual terminal and core structures

Monika Häring  1 , Gisle Vestergaard, Kim Brügger, Reinhard Rachel, Roger A Garrett, David Prangishvili
Affiliations

Structure and genome organization of AFV2, a novel archaeal lipothrixvirus with unusual terminal and core structures

Monika Häring et al. J Bacteriol. 2005 Jun.

Abstract

A novel filamentous virus, AFV2, from the hyperthermophilic archaeal genus Acidianus shows structural similarity to lipothrixviruses but differs from them in its unusual terminal and core structures. The double-stranded DNA genome contains 31,787 bp and carries eight open reading frames homologous to those of other lipothrixviruses, a single tRNA(Lys) gene containing a 12-bp archaeal intron, and a 1,008-bp repeat-rich region near the center of the genome.

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Figures

FIG. 1.
FIG. 1.
Electron micrographs of virions of AFV2 negatively stained with 3% uranyl acetate. A, Native virion. B, Virion after treatment with 0.1% SDS for 1 min. C to E, 3D reconstruction of the termini of a native virion. A and B, Two different horizontal slices (0.7 nm) through the 3D data set. C, Color-coded representation of 3D structure. Pink, virion termini; yellow, negative stain encasing the virion. Bars, 200 nm (A and B) or 100 nm (C to E).
FIG. 2.
FIG. 2.
Scheme of the structure of an AFV2 virion.
FIG. 3.
FIG. 3.
Genome map of AFV2 showing the location and size of the putative genes present on the two DNA strands. Genes are expressed from left to right in the upper row and from right to left in the lower row. ORF homologs that are shared with other Acidianus or Sulfolobus viruses AFV1, SIFV, SIRV1, and SIRV2 are represented by filled rectangles.
See this image and copyright information in PMC

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