replica plating
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replica plating
[′rep·lə·kə ‚plād·iŋ] (microbiology)
A method for the isolation of nutritional mutants in microorganisms; colonies are grown from a microorganism suspension previously exposed to a mutagenic agent, on a complete medium in a petri dish; a velour surface is used to transfer the impression of all these colonies to a petri dish containing a minimal medium; colonies that do not grow on the minimal medium are the mutants.
McGraw-Hill Dictionary of Scientific & Technical Terms, 6E, Copyright © 2003 by The McGraw-Hill Companies, Inc.