2-methylcitrate synthase
| 2-methylcitrate synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.3.3.5 | ||||||||
| CAS no. | 57827-78-8 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
2-methylcitrate synthase (EC 2.3.3.5) is an enzyme that catalyzes the chemical reaction
-2-methylcitric_acid.svg)
The three substrates of this enzyme are oxaloacetic acid, propionyl-CoA, and water. Its two products are a specific stereoisomer of 2-methylcitric acid, with coenzyme A as the byproduct. The enyme has been characterised from Candida lipolytica,[1] Escherichia coli,[2] and Salmonella typhimurium.[3] The isomer produced was shown to be (2R,3S)-2-methylcitric acid.[4] The methyl citrate product in fungi is later converted to methylisocitric acid and then to pyruvic acid and succinic acid by the enzyme methylisocitrate lyase.[5][6]
This enzyme belongs to the family of transferases, specifically those acyltransferases that convert acyl groups into alkyl groups on transfer. The systematic name of this enzyme class is propanoyl-CoA:oxaloacetate C-propanoyltransferase (thioester-hydrolysing, 1-carboxyethyl-forming). Other names in common use include 2-methylcitrate oxaloacetate-lyase, MCS, methylcitrate synthase, and methylcitrate synthetase.[7]
References
[edit]- ↑ Uchiyama H; Tabuchi T (1976). "Properties of methylcitrate synthase from Candida lipolytica". Agric. Biol. Chem. 40 (7): 1411–1418. doi:10.1271/bbb1961.40.1411.
- ↑ Buckel W; Wendisch, VF; De Graaf, AA; Müller, U; Linder, MI; Linder, D; Buckel, W (1997). "Propionate oxidation in Escherichia coli: evidence for operation of a methylcitrate cycle in bacteria". Arch. Microbiol. 168 (5): 428–36. Bibcode:1997ArMic.168..428T. doi:10.1007/s002030050518. PMID 9325432.
- ↑ Horswill AR, Escalante-Semerena JC (1999). "Salmonella typhimurium LT2 catabolizes propionate via the 2-methylcitric acid cycle". J. Bacteriol. 181 (18): 5615–23. doi:10.1128/JB.181.18.5615-5623.1999. PMC 94080. PMID 10482501.
- ↑ Wadman SK; Mienie, LJ; Erasmus, E; De Wet, WJ; Ketting, D; Duran, M; Wadman, SK (1994). "Identification of the stereoisomeric configurations of methylcitric acid produced by si-citrate synthase and methylcitrate synthase using capillary gas chromatography-mass spectrometry". J. Inherit. Metab. Dis. 17 (6): 738–47. doi:10.1007/BF00712017. PMID 7707698.
- ↑ Domin, Nicole; Wilson, Duncan; Brock, Matthias (2009). "Methylcitrate cycle activation during adaptation of Fusarium solani and Fusarium verticillioides to propionyl-CoA-generating carbon sources". Microbiology. 155 (12): 3903–3912. doi:10.1099/mic.0.031781-0. PMID 19661181.
- ↑ Brock, Matthias; Maerker, Claudia; Schütz, Alexandra; Völker, Uwe; Buckel, Wolfgang (2002). "Oxidation of propionate to pyruvate in Escherichia coli". European Journal of Biochemistry. 269 (24): 6184–6194. doi:10.1046/j.1432-1033.2002.03336.x. PMID 12473114.
- ↑ Enzyme 2.3.3.5 at KEGG Pathway Database.
