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Molecular study of Stenoponia tripectinata tripectinata (Siphonaptera: Ctenophthalmidae: Stenoponiinae) from the Canary Islands: taxonomy and phylogeny

Published online by Cambridge University Press:  18 August 2015

A. Zurita ,
R. Callejón ,
M. De Rojas ,
M.S. Gómez López  and
C. Cutillas
A. Zurita
Affiliation:
Department of Microbiology and Parasitology. Faculty of Pharmacy, University of Sevilla, Profesor García González 2, 41012 Sevilla, Spain
R. Callejón
Affiliation:
Department of Microbiology and Parasitology. Faculty of Pharmacy, University of Sevilla, Profesor García González 2, 41012 Sevilla, Spain
M. De Rojas
Affiliation:
Department of Microbiology and Parasitology. Faculty of Pharmacy, University of Sevilla, Profesor García González 2, 41012 Sevilla, Spain
M.S. Gómez López*
Affiliation:
Department of Microbiology and Parasitology. Faculty of Pharmacy, University of Barcelona, Avda, Joan XXIII, 08028 Barcelona, Spain
C. Cutillas*
Affiliation:
Department of Microbiology and Parasitology. Faculty of Pharmacy, University of Sevilla, Profesor García González 2, 41012 Sevilla, Spain
*
* Author for correspondence Phone: +34 95 4556773 Fax: +34 95 4628162 E-mail: cutillas@us.es
* Author for correspondence Phone: +34 95 4556773 Fax: +34 95 4628162 E-mail: cutillas@us.es
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Abstract

In the present work, we carried out a comparative molecular study of Stenoponia tripectinata tripectinata isolated from Mus musculus from the Canary Islands, Spain. The Internal Transcribed Spacers 1 and 2 (ITS1, ITS2) and 18S ribosomal RNA partial gene and cytochrome c-oxidase 1 (cox1) mitochondrial DNA partial gene sequences of this subspecies were determined to clarify the taxonomic status of this subspecies and to assess inter-population variation and inter-specific sequence differences. In addition, we have carried out a comparative phylogenetic study with other species of fleas using Bayesian, Maximum Parsimony, Maximum Likelihood and Neighbor-Joining analysis. A geographical signal was detected between the cox1 partial gene sequences of S. t. tripectinata isolated from M. musculus from different islands and those isolated from Apodemus sylvaticus from the Iberian Peninsula. Our results assess the monophyletic origin of Stenoponiinae and a different genetic lineage from Ctenophthalmidae. Thus, the elevation of subfamily Stenoponiinae to family level (Stenoponiidae) is suggested.

Keywords

Stenoponia tripectinata tripectinataribosomal RNAcytochrome c-oxidase 1Canary IslandsSiphonaptera

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Type
Research Papers
Information
Bulletin of Entomological Research , Volume 105 , Issue 6 , December 2015 , pp. 704 - 711
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Copyright © Cambridge University Press 2015 

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