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Metallo-supramolecular Polymers: Versatile DNA Binding and Their Cytotoxicity

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Abstract

Metallo-supramolecular polymers (RuL1, RuL2, RuL3, FeL3, and FeL4) prepared by complexation of bis(terpyridine) derivatives with Ru2+ or Fe2+ ions with octahedral coordination structures showed high binding ability to several DNAs (calf thymus DNA, herring sperm DNA, [poly(dA-dT)]2, and [poly(dG-dC)]2), which were revealed by UV–Vis absorption titration experiments. The electrostatic interactions between the metal cations of the polymers and phosphate anions of DNA led to formation of conjugate structure. The binding constant observed reached 3.7 × 107 M−1, which is the highest among values reported for metal complexes to date. Based on a long strand structure of the polymer, groove binding is most possible binding mode. Cell viability experiments showed that RuL3 and FeL3 displayed highly statistical significance (**p<0.01) to human non small cell lung cancer cell lines (NCI-H460).

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Acknowledgments

This work was financially supported by the Ministry of Education, Culture, Sports, Sciences, and Technology, Japan.

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Authors and Affiliations

  1. Polymer Materials Unit, National Institute for Materials Science (NIMS), 1-1 Namiki, Tsukuba, 305-0044, Japan

    Jinghua Li & Masayoshi Higuchi

  2. Institute of Integrated Cell-Materials Sciences (iCeMS), Kyoto University, Yoshida-Honmachi, Sakyo-ku, Kyoto, 606-8501, Japan

    Tatsuya Murakami

  3. Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency (JST), 7, Gobancho, Chiyoda-ku, Tokyo, 102-0076, Japan

    Masayoshi Higuchi

Authors
  1. Jinghua Li
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  2. Tatsuya Murakami
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  3. Masayoshi Higuchi
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Corresponding author

Correspondence to Masayoshi Higuchi.

Additional information

This paper is dedicated to Prof. Dr. Hiroshi Nishihara for his outstanding contribution to the field of metal-containing polymers.

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Li, J., Murakami, T. & Higuchi, M. Metallo-supramolecular Polymers: Versatile DNA Binding and Their Cytotoxicity. J Inorg Organomet Polym 23, 119–125 (2013). https://doi.org/10.1007/s10904-012-9752-2

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  • DOI: https://doi.org/10.1007/s10904-012-9752-2

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